As part of the project on Lymnaea, we have invested heavily in next-generation sequencing and bioinformatic resources. The next generation sequencing data, all of which will be released onto GenBank, is made up of 454 EST libraries and Illumina/Hi-Seq short read sequences. Data have been analysed in conjunction with our University of Edinburgh collaborators, and more recently using our own server (2x Quad-Core Xeon processor, 32GB RAM, 500GB and 4 x 1TB hard drives).
We have used ~20-40 fold Illumina/HiSeq and ~1 fold 454 coverage to assemble a first draft of the Lymnaea stagnalis genome. This resource is available here - contact Angus Davison if you would like to arrange guest access.
RAD genotyping data has been analysed using the RAD tools software that Dr John Davey developed as part of the ongoing BBSRC grant. All things RAD are available from the RAD sequencing wiki at Edinburgh.
We collaborated with BioS&T to produce a BAC library for Lymnaea stagnalis. The BAC library is also potentially available for common use - enquiries to Angus Davison. BioS&T have been excellent at every stage of the process and I have no qualms in strongly recommending them to others.
Dr Maureen Liu has recently held two training classes in the use of RADSeq / RAD genotyping, including scientists from the Universities of Sheffield and Manchester. Both have resulted in the successful construction of RAD libraries. Please enquire to Angus Davison if interested.